Related Experiment Videos
A simple assay for formate dehydrogenase activity by gas chromatography-mass spectrometry
T Shiraishi1, E Fukusaki, S Kajiyama
1Department of Biotechnology, Graduate School of Engineering, Osaka University, Japan.
Journal of Chromatography. A
|October 9, 1999
Abstract:
A new method using GC-MS was devised for the convenient measurement of formate dehydrogenase (FDH) activity in crude tissue samples. FDH activity was detected by measuring headspace 13CO2, which was enzymically converted from [13C]formic acid. This method proved to be sensitive and simple for the estimation of FDH activity without complicated pretreatment.