Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Assessment of budesonide patch tests.

B Bjarnason1, E Flosadóttir, T Fischer

  • 1Department of Dermatology, Karolinska Hospital, Stockholm, Sweden.

Contact Dermatitis
|October 9, 1999
PubMed
Summary

Improving budesonide patch testing requires longer application times. Optimal detection of positive reactions in subjects needed 48-hour applications with readings at 96 and 216 hours.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cedalion Tutorial: A Python-based framework for comprehensive analysis of multimodal fNIRS & DOT from the lab to the everyday world.

ArXiv·2026
Same author

Early image acquisition <sup>68</sup>Ga-FAPI-46-PET/CT in prone position is promising in the workup of rectosigmoid and anal carcinoma.

European journal of nuclear medicine and molecular imaging·2025
Same author

Development of a breathing lung phantom for proton CT imaging.

Physics in medicine and biology·2025
Same author

[Fulminant amniotic fluid embolism with subsequent cesarean section under prolonged resuscitation-A case report].

Die Anaesthesiologie·2024
Same author

Prevention of legionella infections from toilet flushing cisterns.

The Journal of hospital infection·2024
Same author

The relevance of reducing Veress needle overshooting.

Scientific reports·2023

Area of Science:

  • Dermatology
  • Allergy and immunology

Background:

  • Patch testing is crucial for diagnosing allergic contact dermatitis.
  • Current budesonide patch test protocols may require optimization for improved sensitivity and specificity.

Purpose of the Study:

  • To evaluate the impact of varying budesonide concentrations and application durations on patch test reactions.
  • To determine optimal reading time points for budesonide patch tests.

Main Methods:

  • 20 subjects with prior budesonide patch test reactions were re-tested with serial doses (0.074-150 microg/cm2) on polyester patches for 3-96 hours.
  • Reactions were assessed visually and with laser Doppler perfusion imaging up to 264 hours.
  • A subset of subjects underwent 48-hour testing with 0.1% budesonide in petrolatum using Finn Chambers.

Main Results:

  • Reaction severity was unpredictably affected by dose and application time; high doses did not suppress reactivity.
  • Optimal reading times varied but were consistent for individuals across methods.
  • Longer than 24-hour applications were needed to detect all positive reactions at 2.0 microg/cm2.
  • 48-hour applications with readings at 96 and 216 hours were optimal for distinguishing positive from negative subjects.

Conclusions:

  • Budesonide patch test sensitivity is enhanced by prolonged application times (48 hours) and specific reading schedules.
  • The polyester patch method with optimized timing appears superior to the Finn Chamber method for budesonide patch testing.

Related Experiment Videos