Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Autoradiographical and behavioural effects of a chronic infusion of antisense to the alpha2D-adrenoceptor in the rat.

E S Robinson1, D J Nutt, L Hall

  • 1Psychopharmacology Unit, School of Medical Sciences, University Walk, Bristol, BS8 1TD, UK.

British Journal of Pharmacology
|October 12, 1999
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Human brain changes after first psilocybin use.

Nature communications·2026
Same author

Serotonergic psychedelics for depression: A comprehensive overview.

International review of neurobiology·2025
Same author

Suicidal Ideation in Medicinal Cannabis Patients: A 12-Month Prospective Study.

Archives of suicide research : official journal of the International Academy for Suicide Research·2024
Same author

Psilocybin and Other Classic Psychedelics in Depression.

Current topics in behavioral neurosciences·2023
Same author

Canalization and plasticity in psychopathology.

Neuropharmacology·2022
Same author

Incidence of hyperoxia in trauma patients receiving pre-hospital emergency anaesthesia: results of a 5-year retrospective analysis.

Scandinavian journal of trauma, resuscitation and emergency medicine·2021

Antisense oligonucleotides selectively reduced alpha2D-adrenoceptor expression, attenuating hypothermia and sedative effects of UK 14,304 in rats. These changes were reversible after treatment cessation.

Area of Science:

  • Neuropharmacology
  • Molecular Biology

Background:

  • Alpha2-adrenoceptors play critical roles in physiological and behavioral processes.
  • Understanding the specific functions of alpha2D-adrenoceptor subtypes is essential for targeted drug development.

Purpose of the Study:

  • To investigate the effects of antisense oligonucleotides targeting alpha2D-adrenoceptor on receptor binding.
  • To assess the in vivo impact of chronic antisense infusion on alpha2-adrenoceptor function, including behavioral and physiological parameters.

Main Methods:

  • Receptor autoradiography was used to quantify [3H]-RX821002 binding following antisense treatment.
  • Behavioral and physiological responses, specifically hypothermia and sedative effects induced by UK 14,304, were measured.
  • Specificity of the antisense approach was confirmed by examining beta-adrenoceptor expression.

Related Experiment Videos

Main Results:

  • A 3-day infusion of alpha2D-adrenoceptor antisense significantly reduced receptor binding in the septum and anterior hypothalamic area by 20-30%.
  • Antisense treatment attenuated the hypothermic and sedative responses to UK 14,304 on the second day of infusion.
  • These effects were fully reversible, with normal responses observed 4 days post-infusion.

Conclusions:

  • Antisense oligonucleotides effectively and selectively reduced alpha2D-adrenoceptor expression in specific brain regions.
  • Knockdown of alpha2D-adrenoceptors leads to a significant attenuation of UK 14,304-induced hypothermia and sedative actions.
  • The observed functional changes were transient and fully reversible upon cessation of antisense treatment.