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A multicopper oxidase gene from Candida albicans: cloning, characterization and disruption
Raimund Eck1, Susanne Hundt1, Albert Härtl1
1Hans-Knöll-Institut für Naturstoff-Forschung eV, Abteilung Mikrobielle Infektionsbiologie, Beutenbergstrasse 11,D-07745 Jena, Germany1.
Abstract:
A multicopper oxidase gene from the human pathogenic yeast Candida albicans was isolated and characterized. An open reading frame of 1872 bp, designated CaFET3, was identified, encoding a predicted protein of 624 amino acids and a molecular mass of 70.5 kDa. The identity between the deduced amino acid sequences of CaFET3 and the Saccharomyces cerevisiae FET3 gene is 55%. CaFET3 was localized on chromosome 6. A null mutant (fet3delta/fet3delta) was constructed by sequential gene disruption. Unlike the C. albicans SC5314 wild-type strain the fet3delta mutant was unable to grow in low-iron medium. The lack of growth of a S. cerevisiae fet3delta mutant in iron-limited medium was compensated by transformation with CaFET3. The null mutant strain showed no change in pathogenicity compared with the wild-type strain in the mouse model of systemic candidiasis.