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Related Experiment Videos

SF/HGF is a mediator between limb patterning and muscle development.

M Scaal1, A Bonafede, V Dathe

  • 1Institute of Anatomy, University of Freiburg, Albertstrasse 17, D-79104 Freiburg, Germany. brandsab@uni-freiburg.de.

Development (Cambridge, England)
|October 16, 1999
PubMed
Summary

Scatter factor/hepatocyte growth factor (SF/HGF) guides muscle precursor cell migration and patterning in developing limbs. Its expression is regulated by limb patterning signals, influencing cell movement and differentiation.

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Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Cell Biology

Background:

  • Scatter factor/hepatocyte growth factor (SF/HGF) is implicated in early myogenic precursor cell migration.
  • The role of persistent SF/HGF expression in later limb bud development remains unclear.

Purpose of the Study:

  • To investigate the regulation of SF/HGF expression during limb bud development.
  • To determine the influence of SF/HGF on myogenic precursor cell direction and patterning within the limb bud mesenchyme.

Main Methods:

  • Analysis of SF/HGF expression in response to limb patterning manipulations (AER removal, ZPA excision).
  • Assessment of FGF-2 and BMP-2 effects on SF/HGF expression.
  • Tracking of Pax-3-positive cells following ZPA excision.

Related Experiment Videos

  • Evaluation of SF/HGF's impact on myogenic precursor cell motility and differentiation in vitro.

    • Main Results:

    • SF/HGF expression is dependent on apical ectodermal ridge (AER) signals and can be rescued by FGF-2.
    • Zone of polarizing activity (ZPA) excision leads to ectopic SF/HGF expression, potentially inhibited by BMP-2.
    • ZPA excision causes posterior migration of Pax-3-positive cells, suggesting a role in premuscle mass positioning.
    • SF/HGF enhances myogenic precursor cell motility and maintains their undifferentiated state during migration.

    Conclusions:

    • SF/HGF expression is tightly regulated by limb patterning cues.
    • SF/HGF plays a critical role in directing myogenic precursor cell migration and patterning within the developing vertebrate limb.