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The yeast Saccharomyces cerevisiae does not sequester chloride but can express a functional mammalian chloride
L A Coury1, J E McGeoch, G Guidotti
1Renal Electrolyte Division, Department of Medicine, University of Pittsburgh Medical Center, Pittsburgh, PA 15213-2500, USA.
Abstract:
Chloride uptake into yeast was measured as a function of pH. A small amount of uptake was seen at pH values of 3.0 and 4.0; at pH 6.0 chloride uptake was substantially less than the uptake of phosphate and rubidium. Because chloride uptake is inefficient, we expressed the putative mammalian chloride channel, pI(Cln), in yeast and observed a chloride-selective current when total membrane protein was reconstituted into lipid bilayers. The current was inhibited by a specific chloride channel blocker, 5-nitro-2-(3-phenylpropylamino)-benzoic acid. These results suggest that yeast may serve as a means to characterize chloride channels from other organisms.