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A Rapid and Efficient Method to Dissect Pupal Wings of Drosophila Suitable for Immunodetections or PCR Assays
Published on: December 30, 2017
[Duplications of the wing-hinge structures in the Pth mutants of drosophila melanogaster]
E M Khovanova1, I N Latyshev, E G Ugnivenko
1Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia.
Abstract:
The Pth mutation of Drosophila melanogaster, a unique dominant mutation obtained by us, causes the notum duplications that are followed by the duplications of ventral and dorsal wing-hinge structures. The duplications of the wing hinge have a strictly coordinated structure, can be ranged in a continuous series, and are divided into four distinct types, none of which overlaps with the other. The order of the emergence of the ventral wing-hinge structures was determined in duplication forms, and the shape, size, and location of these structures were compared with normal parameters. The growth of the presumptive wing-hinge region was shown to have a vectorial mode; the directions of the main vectors and their center were determined. A geometrical model is proposed, which adequately explains the strict specificity in the structure of duplications as well as the agreement between the duplication forms to one another in each of the four types.
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