Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Platelet glycoprotein (GP) V polymorphisms in Japanese.

S Kunishima1, T Shimizu, T Kamiya

  • 1Japanese Red Cross Aichi Blood Center, Seto. kunisima@fujita-hu.ac.jp

Tissue Antigens
|October 16, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Acquisition of interleukin-3 independence in FDC-P2 cells after transfection with the activated c-H-ras gene using a bovine papillomavirus-based plasmid vector.

Blood·1992
Same author

Adult T-cell leukemia developing during immunosuppressive treatment in a renal transplant recipient.

American journal of hematology·1992
Same author

An increase in spontaneous proliferation of peripheral blood mononuclear cells in the early phase after liver transplantation--a useful indication of acute rejection.

Transplantation·1992
Same author

[A case of spontaneous mediastinal emphysema developed by numbness of the left arm, dysphagia and chest pain].

Kokyu to junkan. Respiration & circulation·1992
Same author

An appraisal of pediatric liver transplantation from living relatives. Initial clinical experiences in 20 pediatric liver transplantations from living relatives as donors.

Annals of surgery·1992
Same author

Tolerance of the liver as measured by ketone body ratio and hepatocyte energy charge to 120 minutes acute venous congestion in dogs.

Circulatory shock·1992
Same journal

Corrigendum.

Tissue antigens·2015
Same journal

Nomenclature for factors of the HLA system, update September 2015.

Tissue antigens·2015
Same journal

Nomenclature for factors of the HLA system, update August 2015.

Tissue antigens·2015
Same journal

Nomenclature for factors of the HLA system, update July 2015.

Tissue antigens·2015
Same journal

Four amino acid exchanges located in the alpha-2 domain specify the novel HLA-B*50:20 allele.

Tissue antigens·2015
Same journal

A novel HLA-A*02 variant, HLA-A*02:575, detected in a Taiwanese bone marrow hematopoietic stem cell donor.

Tissue antigens·2015
See all related articles

Researchers identified gene frequencies for glycoprotein V (GPV) polymorphisms in Japanese and Caucasian populations. The 341Gly/Arg variant is common and expressed on platelets, suggesting its potential role as a platelet alloantigen.

Area of Science:

  • Genetics
  • Immunology
  • Hematology

Background:

  • Glycoprotein (GP) V is a key component of the platelet GPIb/IX/V complex, crucial for von Willebrand factor binding.
  • Recent discovery of GPV gene polymorphisms, including non-synonymous and silent variants, necessitates population-based frequency analysis.

Purpose of the Study:

  • To determine the gene frequencies of non-synonymous GPV polymorphisms in Japanese and Caucasian populations.
  • To investigate the expression of GPV variants on platelet surfaces.

Main Methods:

  • Development of an allele-specific restriction method for genotyping GPV polymorphisms.
  • Genotyping of Japanese and Caucasian individuals.
  • Flow cytometric analysis to assess platelet surface expression of GPV variants.

Related Experiment Videos

Main Results:

  • The 341Gly/Arg polymorphism showed high frequencies in both populations (Japanese: 0.94 Gly, 0.06 Arg; Caucasian: 0.95 Gly, 0.05 Arg).
  • Rare variants at positions 114Asp/Tyr, 273Met/Ile, and 397Leu/Arg were not detected.
  • The 341Arg variant was confirmed to be normally expressed on platelet membranes.

Conclusions:

  • The 341Gly/Arg polymorphism is a common genetic variation in GPV within Japanese and Caucasian populations.
  • Normal expression of the 341Arg variant suggests its potential significance as a platelet alloantigen, relevant for transfusion medicine and immunology.