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Updated: Jul 27, 2026
Optimized Ex-ovo Culturing of Chick Embryos to Advanced Stages of Development
Published on: January 24, 2015
Development and integration of an extended embryo culture program
P E Patton1, K Sadler-Fredd, K A Burry
1Department of Obstetrics and Gynecology, Oregon Health Sciences University, Portland, USA.
Objective:
To study and evaluate a sequential, extended embryo culture system.
Design:
Prospective study.
Setting:
University-affiliated IVF clinic.
Patient(S):
All couples who were treated between October 1997 and July 1998.
Intervention(S):
A standard human tubal fluid plus 10% serum substitute supplement (SSS) culture medium was used. The embryos were transferred to extended culture medium (S2 or G2) on day 3.
Main Outcome Measure(S):
Blastocyst formation and implantation and pregnancy rates.
Result(S):
Forty percent of the 20 donated cryopreserved embryos progressed to the blastocyst stage by day 6. Clinically, 7 (5.6%) of the 125 cycles did not result in a transfer. Blastocyst formation rates ranged from 33%-63% in the five study groups. Implantation rates ranged from 15%-52% and pregnancy rates ranged from 37%-75%.
Conclusion(S):
Extended culture to day 5 or 6 results in acceptable blastocyst formation rates, implantation rates, and pregnancy rates.
