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Updated: Oct 19, 2026

Mouse Round Spermatid Injection
Published on: January 26, 2024
Birth of a healthy infant after conception with round spermatids isolated from cryopreserved testicular tissue
L Gianaroli1, H A Selman, M C Magli
1Reproductive Medicine Unit, S.I.S.ME.R., Bologna, Italy. sismer@iol.it
Objective:
To report a case of nonobstructive azoospermia in which round spermatids recovered from thawed testicular tissue were used for injection.
Design:
Case report.
Setting:
Reproductive Medicine Unit, S.I.S.ME.R.
Patient(S):
A 33-year-old azoospermic man.
Intervention(S):
Intracytoplasmic sperm injection with frozen-thawed spermatids.
Main Outcome Measure(S):
Fertilization, embryo cleavage, pregnancy, and delivery.
Result(S):
Birth of a healthy, chromosomally normal girl.
Conclusion(S):
Frozen-thawed testicular round spermatids from a patient with a history of incomplete spermatogenesis can maintain their viability and their capacity to fertilize and to lead to full-term pregnancy.
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