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Related Experiment Videos

Human basophils express CD22 without expression of CD19.

K Han1, Y Kim, J Lee

  • 1Department of Clinical Pathology, Catholic University Medical College, Seoul, Korea. hankja@cmc.cuk.ac.kr

Cytometry
|October 16, 1999
PubMed
Summary

Flow cytometry using CD22+/CD19- markers provides a precise method for counting basophils. This technique offers a reliable and accurate alternative to current automated cell counters for clinical applications.

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Area of Science:

  • Hematology
  • Immunology
  • Cell Biology

Background:

  • Modern automated cell counters struggle with precise basophil enumeration.
  • Accurate and efficient basophil counting methods are crucial for clinical diagnostics.

Purpose of the Study:

  • To develop and validate a rapid, accurate, and precise flow cytometry method for counting basophils.
  • To identify specific cell surface markers for reliable basophil identification.

Main Methods:

  • Flow cytometry was employed to count basophils (CD22+/CD19-) and B cells (CD22+/CD19+).
  • Two analysis methods were used: gating on lymphocytes (%basophils (G%baso)) and direct region analysis (%basophils (R1%baso)).
  • Flow cytometric counts were compared with manual and automated counts in normal controls and chronic myelogenous leukemia (CML) patients.

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Main Results:

  • Both flow cytometry methods (G%baso and R1%baso) showed high correlation (r=0.996) and were well-correlated with manual counts (r=0.827-0.831).
  • The CD22+/CD19- marker combination accurately identified basophils (99.48% purity) with characteristic CD13, CD33, and dim CD45 expression.
  • Automated cell counting demonstrated the poorest correlation with manual basophil counts (r=0.692).

Conclusions:

  • The CD22+/CD19- marker combination is a specific identifier for basophils.
  • Flow cytometry using these markers offers an easy, reliable, and accurate method for basophil counting.
  • This method presents a significant improvement over current automated cell counting techniques.