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Bovine ornithine decarboxylase gene: cloning, structure and polymorphisms
J Yao1, D Zadworny, S E Aggrey
1Dept. of Animal Science, McGill University, Quebec, Canada.
DNA Sequence : the Journal of DNA Sequencing and Mapping
|October 16, 1999
Summary
Researchers sequenced the bovine ornithine decarboxylase (ODC) gene, revealing high conservation across species. They identified key regulatory elements and developed methods to detect genetic variations in the ODC gene.
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Background:
- The ornithine decarboxylase (ODC) gene is crucial for cell growth and proliferation.
- Understanding the genetic structure of bovine ODC is important for livestock research.
Purpose of the Study:
- To isolate and characterize the bovine ornithine decarboxylase (ODC) gene.
- To analyze the gene's structure, regulatory elements, and conservation.
Main Methods:
- Genomic library screening using bacteriophage lambda DASH.
- DNA sequencing and sequence analysis.
- Primer extension analysis and PCR-based polymorphism detection.
Main Results:
- The complete 9452 bp bovine ODC gene sequence was determined, comprising 12 exons.
- High conservation was observed between bovine, human, and mouse ODC genes.
- Key regulatory elements (TATA box, CCAAT, GC boxes, CRE) and transcription start site were identified in the 5'-flanking region.
- Two polymorphic restriction sites (TaqI and MspI) were mapped, with PCR methods developed for their detection.
Conclusions:
- The bovine ODC gene structure is highly conserved, suggesting functional importance.
- Identified regulatory elements provide insights into gene expression control.
- Developed polymorphic markers can be utilized for genetic studies in cattle.