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Isolation and identification by sequence homology of a second putative C5-DNA-methyltransferase gene from Ascobolus
1Institut de Génétique et Microbiologie, CNRS/Université Paris-Sud, Orsay, France.
DNA Sequence : the Journal of DNA Sequencing and Mapping
|October 16, 1999
Abstract:
I report the cloning of a new Ascobolus gene (masc 2) that potentially encodes a C5-DNA-methyltransferase. The putative protein exhibits the two domains characteristic of eukaryotic maintenance DNA-methyltransferase: a large N-terminal domain and a C-terminal domain containing all ten catalytic motifs arranged in the canonical order. A new type of eukaryotic DNA-methylase gene (masc 1) has been recently found in Ascobolus. Masc1 is essential for the de novo methylation and dispensable for methylation maintenance. The masc2 gene could encode the methylase involved in this maintenance.