Assembly of a complete zebrafish mitochondrial 16S rRNA gene from overlapping expressed sequence tags

A complete zebrafish mitochondrial 16S ribosomal RNA gene was assembled from our existing zebrafish EST clones by aligning them with the carp mitochondria 16S rRNA sequence. The overall homology between our assembled zebrafish mt 16S rRNA and the carp mt 16S rRNA is 83.5%. As the number of zebrafish ESTs grows, the assembly of more full-length cDNA sequences from overlapping EST data could be expected.