Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Tissue-specific p53 expression in the nervous system.

Y Tendler1, G Weisinger, R Coleman

  • 1Department of Clinical Biochemistry, Rambam Medical Center, P.O. Box 9602, Haifa, Israel. tendler@tx.technion.ac.il

Brain Research. Molecular Brain Research
|October 16, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diurnal variations of androgens in sexually mature male Bolivian squirrel monkeys (Saimiri sciureus) during the breeding season.

American journal of primatology·2020
Same author

Cytotoxic chemotherapy in the treatment of advanced renal cell carcinoma in the era of targeted therapy.

Critical reviews in oncology/hematology·2015
Same author

12S-Lipoxygenase is necessary for human vascular smooth muscle cell survival.

Experimental cell research·2013
Same author

Anti-thyroid cancer properties of a novel isoflavone derivative, 7-(O)-carboxymethyl daidzein conjugated to N-t-Boc-hexylenediamine in vitro and in vivo.

The Journal of steroid biochemistry and molecular biology·2011
Same author

Atypical fibroxanthoma of the scalp following hair transplantation in a 35-year-old male.

Journal of plastic, reconstructive & aesthetic surgery : JPRAS·2010
Same author

Inducible preproenkephalin DNA binding proteins in the rat striatum.

Molecular and cellular neurosciences·2009
Same journal

Adenoviral interneuronal transportation after retrograde gene transfer in mice.

Brain research. Molecular brain research·2005
Same journal

IGF-I protects cortical neurons against ceramide-induced apoptosis via activation of the PI-3K/Akt and ERK pathways; is this protection independent of CREB and Bcl-2?

Brain research. Molecular brain research·2005
Same journal

Scutellariae radix extracts suppress ethanol-induced caspase-11 expression and cell death in N(2)a cells.

Brain research. Molecular brain research·2005
Same journal

Two estrogen replacement therapies differentially regulate expression of estrogen receptors alpha and beta in the hippocampus and cortex of ovariectomized rat.

Brain research. Molecular brain research·2005
Same journal

Molecular regulation of glutamate and GABA transporter proteins by clobazam during epileptogenesis in Fe(+++)-induced epileptic rats.

Brain research. Molecular brain research·2005
Same journal

Partial cloning of neuronal nitric oxide synthase (nNOS) cDNA and regional distribution of nNOS mRNA in the central nervous system of the Nile tilapia Oreochromis niloticus.

Brain research. Molecular brain research·2005
See all related articles

This study reveals significant p53 transcription factor activity in the nervous system and eyes of transgenic mice. Unexpectedly high p53 expression was found in non-dividing cells within the brain and eyes.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • The p53 transcription factor is crucial for regulating cell proliferation and apoptosis.
  • Previous studies indicated p53 promoter activity primarily in testes of p53-CAT transgenic mice.

Purpose of the Study:

  • To investigate p53 expression patterns in the central and peripheral nervous systems.
  • To analyze p53 activity in both transgenic and parental mouse strains.

Main Methods:

  • Utilized p53 promoter-driven bacterial chloramphenicol acetyl transferase (CAT) reporter gene expression.
  • Employed p53 pan ELISA assays for protein quantification.
  • Conducted immunohistochemical studies for detailed expression localization.

Main Results:

Related Experiment Videos

  • High p53 promoter-driven CAT activity was detected in the cerebellum, hippocampus, hypothalamus, pons, thalamus, and upper spinal cord.
  • Unexpectedly high CAT expression and p53 protein levels were observed in the eyes (cornea, retina).
  • Significant p53 activity was found in post-mitotic, non-dividing cells.

Conclusions:

  • p53 exhibits widespread expression in the mouse nervous system and eyes.
  • The findings highlight a novel role for p53 in neuronal and ocular tissues.
  • p53 expression occurs in both dividing and non-dividing cells within these tissues.