Related Experiment Videos
Cloning, structural characterization, and chromosomal localization of the gene encoding the human prostaglandin E(2)
S L Smock1, L C Pan, T A Castleberry
1Department of Cardiovascular and Metabolic Diseases, Central Research Division, Pfizer, Inc., Eastern Point Road, Groton, CT, USA.
Gene
|October 16, 1999
Summary
Researchers investigated the human prostaglandin E(2) (PGE(2)) EP2 receptor gene, finding it has two exons and a single copy on chromosome 14. The atypical placental transcript likely originates from a different gene.
Area of Science:
- Molecular Biology
- Genetics
- Human Physiology
Background:
- Northern blot analysis revealed an unusual low molecular weight transcript in human placenta.
- This transcript was detected using a probe for the human prostaglandin E(2) (PGE(2)) EP2 receptor subtype.
Purpose of the Study:
- To clone and characterize the gene encoding the human PGE(2) EP2 receptor subtype.
- To identify transcriptional initiation and termination sites.
- To determine the gene's chromosomal localization.
Main Methods:
- Gene cloning and characterization.
- Identification of transcriptional start and end sites in spleen and thymus.
- Southern and fluorescence in situ hybridization for chromosomal localization.
Main Results:
- The human EP2 gene has two exons separated by a large intron.
- A common transcriptional initiation site was identified in spleen and thymus.
- Transcript termini were mapped in spleen and thymus; the gene is a single copy located on chromosome 14q22.
Conclusions:
- The human EP2 gene structure and transcription initiation/termination sites were elucidated.
- The gene is localized to chromosome 14q22.
- The atypical placental transcript likely originates from a separate, related gene, not differential splicing of the EP2 gene.