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Related Experiment Videos

Zwittermicin A biosynthetic cluster.

E A Stohl1, J L Milner, J Handelsman

  • 1Department of Plant Pathology, University of Wisconsin-Madison, 1630 Linden Drive, Madison, WI 53706, USA.

Gene
|October 16, 1999
PubMed
Summary
This summary is machine-generated.

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Researchers identified the gene cluster responsible for producing zwittermicin A, a novel antibiotic from Bacillus cereus. This discovery reveals the first biosynthetic pathway for an aminopolyol antibiotic, advancing our understanding of microbial drug production.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Biochemistry

Background:

  • Bacillus cereus produces zwittermicin A, a broad-spectrum aminopolyol antibiotic.
  • Understanding the biosynthesis of novel antibiotics is crucial for drug discovery.

Purpose of the Study:

  • To identify the biosynthetic gene cluster for zwittermicin A in Bacillus cereus.
  • To elucidate the roles of specific genes in zwittermicin A production and resistance.

Main Methods:

  • DNA sequencing of the region flanking the zwittermicin A resistance gene (zmaR).
  • Identification and analysis of open reading frames (ORFs).
  • Insertional inactivation of genes to determine their function.

Main Results:

Related Experiment Videos

  • Three ORFs were identified, with two showing similarity to enzymes involved in polyketide synthesis.
  • orf2 was essential for zwittermicin A production.
  • zmaR was necessary for high-level resistance but not production.
  • ZmaR expression correlated with zwittermicin A production.

    • Conclusions:

    • The identified genes, including zmaR, form a cluster involved in zwittermicin A biosynthesis.
    • This represents the first described biosynthetic pathway for an aminopolyol antibiotic.
    • The findings provide a foundation for understanding and engineering novel antibiotic production.