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Anoxia/induced membrane changes in human red blood cells
A Nicák1, J Mojzis, M Jandoseková
1Department of Pharmacology, Medical Faculty, Safárik University, Slovak Republic. mojzis@central.medic.upjs.sk
Abstract:
The cation-osmotic hemolysis was studied in human red blood cells incubated under anoxic conditions. In relation to the time course of anoxia, two phases of hemolysis were distinguished. A significant decrease of hemolysis was found between 3 and 24 h of incubation. On the other hand, hemolysis was significantly increased after prolonged incubation (48-72 h). Using the method of cation-osmotic hemolysis, the properties of two membrane constituents, spectrine membrane skeleton and membrane bilayer, were studied. The relation between cation-osmotic hemolysis and erythrocyte deformability is being discussed.