Related Experiment Video
Updated: Oct 9, 2026

Preparation of Developing and Adult Drosophila Brains and Retinae for Live Imaging
Published on: March 15, 2010
The rough deal protein is a new kinetochore component required for accurate chromosome segregation in Drosophila
F Scaërou1, I Aguilera, R Saunders
1C.N.R.S., Centre de Génétique Moléculaire, Avenue de la Terrasse, France.
Abstract:
Mutations in the rough deal (rod) gene of Drosophila greatly increase the missegregation of sister chromatids during mitosis, suggesting a role for this gene product in spindle or kinetochore function. The activity provided by rod also appears to be necessary for the recruitment of two known kinetochore components, Zw10 and cytoplasmic dynein. In this paper we describe the cloning of rough deal and an initial cytological characterization of its product. The Rod protein shares no identifiable structural motif with other known proteins, although apparent homologs exist in the genomes of nematode and man. By immunocytochemistry we show that Rod displays a dynamic intracellular staining pattern, localizing first to kinetochores in prometaphase, but moving to kinetochore microtubules at metaphase. Early in anaphase the protein is once again restricted to the kinetochores, where it persists until the end of telophase. This behavior is in all respects similar to that described for Zw10, and suggests that the proteins function together.
More Related Videos
13:59Studying Mitotic Checkpoint by Illustrating Dynamic Kinetochore Protein Behavior and Chromosome Motion in Living Drosophila Syncytial Embryos
Published on: June 14, 2012
11:09Generating a "Humanized" Drosophila S2 Cell Line Sensitive to Pharmacological Inhibition of Kinesin-5
Published on: January 20, 2016
Related Concept Videos
The Ratio of X Chromosome to Autosomes
Normal male Drosophila has a ratio of one X chromosome to two sets of autosomes. In contrast, normal female Drosophila...
Attachment of Sister Chromatids
Forces Acting on Chromosomes
Microtubules and motor proteins exert two types of forces on...
Separation of Sister Chromatids
At the onset of anaphase, separase, a proteolytic enzyme, is...
The Spindle Assembly Checkpoint
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
Meiosis II
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...