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A Noninvasive Hair Sampling Technique to Obtain High Quality DNA from Elusive Small Mammals
Published on: March 13, 2011
Molecular markers reveal exclusively clonal reproduction in Trichophyton rubrum
Y Gräser1, J Kühnisch, W Presber
1Department of Microbiology and Hygiene (Charité-Virchow), Humboldt University, Berlin, Germany. yvonne.graeser@charite.de
Abstract:
Genotypic variability among 96 Trichophyton rubrum strains which displayed different colony morphologies and were collected from four continents was investigated. Twelve markers representing 57 loci were analyzed by PCR fingerprinting, amplified fragment length polymorphism, and random amplified monomorphic DNA markers. Interestingly, none of the methods used revealed any DNA polymorphism, indicating a strictly clonal mode of reproduction and a strong adaptation to human skin.

