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Novel method for rapid determination of clarithromycin sensitivity in Helicobacter pylori
J R Gibson1, N A Saunders, B Burke
1Helicobacter Reference Unit, Laboratory of Enteric Pathogens, Central Public Health Laboratory, London NW9 5HT, United Kingdom.
Journal of Clinical Microbiology
|October 19, 1999
Abstract:
A novel PCR-hybridization assay, performed in single closed capillaries, was developed to detect clarithromycin resistance-associated gene mutations in Helicobacter pylori. Mutations were detected by thermal analysis in 33 of 34 (97%) resistant isolates but not in 66 isolates determined to be sensitive by conventional antibiotic assays. The method was rapid and reproducible, and it reduced PCR product contamination risk.