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A unique bFGF-responsive transcriptional element.

M A Mohideen1, A Hruska-Hageman, M Nilsen-Hamilton

  • 1Department of Biochemistry and Biophysics and the Molecular, Cellular, Iowa State University, Ames 50011, USA.

Gene
|October 19, 1999
PubMed
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Basic fibroblast growth factor (bFGF) activates mrp3 gene transcription via a novel FGF-regulatory element (FRE). This element, identified in 3T3 cells, drives bFGF-responsive gene expression, suggesting a role in regulating mrp3 and other FGF-target genes.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Mitogen-regulated protein/proliferin (mrp/plf) genes encode proteins involved in cell proliferation and angiogenesis.
  • Basic fibroblast growth factor (bFGF) stimulates mrp/plf mRNA and protein production in 3T3 cells.

Purpose of the Study:

  • To identify the minimal promoter sequence responsible for bFGF-responsive transcription of the mrp3 gene.
  • To characterize the FGF-regulatory element (FRE) within the mrp3 promoter.

Main Methods:

  • Truncated mrp3 promoter sequences were analyzed to determine the minimal bFGF-responsive region.
  • The identified FRE was tested for its ability to confer bFGF responsiveness when placed upstream of a thymidine kinase basal promoter.

Main Results:

Related Experiment Videos

  • A minimal bFGF-responsive fragment containing a putative FGF-regulatory element (FRE) was identified within the mrp3 promoter.
  • Nuclear factors in 3T3 cells bind to the FRE.
  • The FRE demonstrated high transcriptional activity and bFGF responsiveness when linked to a heterologous promoter.

Conclusions:

  • The FRE is a potent transcriptional element regulated by bFGF.
  • The FRE likely plays a role in the transcriptional regulation of the mrp3 gene.
  • The FRE may also regulate other FGF-responsive genes.