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[Detection of drug-drug interaction by ESR spectroscopy]
1Department of Dental Pharmacology, Meikai University School of Dentistry, Saitama, Japan.
Abstract:
Vitamin C (sodium ascorbate), gallic acid and dopamine induced apoptosis (characterized by internucleosomal DNA cleavage, nuclear fragmentation, chromatin condensation near nuclear membrane and loss of cell surface microvilli) in human promyelocytic leukemic HL-60 cells. ESR spectroscopy demonstrated the connection between the radical intensity and apoptosis-inducing activity of derivatives of these compounds. When vitamin C and gallic acid were mixed together, the radical intensity and cytotoxic activity of gallic acid was completely scavenged by one or two orders lower concentrations of vitamin C, suggesting the predominant action of vitamin C over gallic acid. When vitamin C and dopamine were mixed together, their radical intensity and apoptosis-inducing activity counteracted with each other. On the other hand, lignins significantly enhanced both the radical intensity and apoptosis-inducing activity of vitamin C. ESR spectroscopy might be applicable for the detection of drug-drug interaction.
Insights
Vitamin C, gallic acid, and dopamine induce apoptosis in leukemia cells. Vitamin C dominates gallic acid interactions, while lignins enhance vitamin C
Area of Science:
- Biochemistry
- Cell Biology
- Pharmacology
Context:
- Investigates the mechanisms of apoptosis induction by Vitamin C (sodium ascorbate), gallic acid, and dopamine in human promyelocytic leukemia HL-60 cells.
- Utilizes Electron Spin Resonance (ESR) spectroscopy to correlate radical intensity with apoptosis-inducing activity.
Purpose:
- To elucidate the interactions between Vitamin C, gallic acid, and dopamine in inducing apoptosis.
- To explore the potential of ESR spectroscopy in detecting drug-drug interactions.
Summary:
- Vitamin C, gallic acid, and dopamine were found to induce apoptosis in HL-60 cells, characterized by specific cellular changes.
- ESR spectroscopy revealed that Vitamin C significantly counteracts or enhances the activity of gallic acid and dopamine, depending on the combination.
- Lignins were observed to potentiate the radical intensity and apoptosis-inducing effects of Vitamin C.
Impact:
- Provides insights into the complex interactions of common compounds in inducing programmed cell death.
- Suggests Vitamin C's predominant role in mixtures with gallic acid.
- Highlights the potential of ESR spectroscopy as a tool for predicting drug-drug interactions and their impact on cellular processes.