Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Spontaneous conversions to aldosterone.

A Aragonés, C P Lantos, G A Locascio

    Acta Physiologica Latino Americana
    |January 1, 1976
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Identification and reproducibility of diagnostic DNA markers for tuber starch and yield optimization in a novel association mapping population of potato (Solanum tuberosum L.).

    TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2016
    Same author

    A non-pathogenic pseudoautosomal region 1 copy number variant downstream of SHOX.

    American journal of medical genetics. Part A·2011
    Same author

    Clinical and molecular evaluation of SHOX/PAR1 duplications in Leri-Weill dyschondrosteosis (LWD) and idiopathic short stature (ISS).

    The Journal of clinical endocrinology and metabolism·2010
    Same author

    Permanent neonatal diabetes caused by a homozygous nonsense mutation in the glucokinase gene.

    Pediatric diabetes·2008
    Same author

    The majority of cases of neonatal diabetes in Spain can be explained by known genetic abnormalities.

    Diabetic medicine : a journal of the British Diabetic Association·2007
    Same author

    Molecular mechanism of activation and nuclear translocation of the mineralocorticoid receptor upon binding of pregnanesteroids.

    Molecular and cellular endocrinology·2004

    Corticosterone conversion to aldosterone is inhibited by boiled adrenal tissue, but a related steroid, 18-hydroxycorticosterone, is not affected. This suggests a specific inhibitory factor present in adrenal tissue.

    Area of Science:

    • Endocrinology
    • Steroid biochemistry

    Background:

    • Aldosterone is a crucial mineralocorticoid hormone regulating blood pressure and electrolyte balance.
    • Understanding the biosynthesis of aldosterone is vital for comprehending adrenal function and related disorders.

    Purpose of the Study:

    • To investigate the precursor roles of corticosterone and a derivative of 18-hydroxycorticosterone in aldosterone synthesis.
    • To identify potential regulatory factors influencing aldosterone production in adrenal tissue.

    Main Methods:

    • Steroid precursors, corticosterone (B) and a less polar 18-hydroxycorticosterone derivative (L), were incubated under three conditions: buffer alone (C), buffer with boiled adrenal tissue (phi), and buffer with surviving quartered rat adrenals (G).
    • Aldosterone yields were quantified from each incubation condition to assess precursor conversion efficiency.

    Related Experiment Videos

    Main Results:

    • Corticosterone (B) showed significantly reduced aldosterone yields in the presence of boiled adrenal tissue (0.21%) compared to buffer alone (1.24%) or surviving adrenals (5.03%).
    • The 18-hydroxycorticosterone derivative (L) exhibited consistent aldosterone yields across all conditions (2.66% in C, 2.30% in phi, 2.47% in G), indicating resistance to inhibition.
    • Boiled adrenal tissue contained a factor that specifically inhibited the conversion of corticosterone to aldosterone, but not that of the 18-hydroxycorticosterone derivative.

    Conclusions:

    • A heat-labile inhibitory factor present in adrenal tissue significantly impairs corticosterone's conversion to aldosterone.
    • The 18-hydroxycorticosterone derivative is a more efficient precursor to aldosterone and is unaffected by this inhibitory factor.
    • These findings shed light on the complex regulation of aldosterone biosynthesis and the specific roles of different steroid precursors.