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Electrokinetic chromatography.

J P Quirino1, S Terabe

  • 1Faculty of Science, Himeji Institute of Technology, Hyogo, Japan.

Journal of Chromatography. A
|October 20, 1999
PubMed
Summary
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Electrokinetic chromatography, particularly with micellar pseudostationary phases, offers advanced separation techniques. Future developments focus on improved detection sensitivity and on-line coupling with mass spectrometry for enhanced analysis.

Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Electrokinetic chromatography (EKC) is a powerful separation technique.
  • Micellar electrokinetic chromatography (MEKC) utilizes micelles as pseudostationary phases, offering unique separation capabilities.

Purpose of the Study:

  • To critically review the key features of electrokinetic chromatography.
  • To highlight advancements in micellar systems and on-line coupling.
  • To discuss future prospects in the field.

Main Methods:

  • Critical review of existing literature on electrokinetic chromatography.
  • Focus on micellar systems and their role as pseudostationary phases.
  • Exploration of on-line coupling techniques, including mass spectrometry.

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Main Results:

  • Detailed overview of separation mechanisms in EKC.
  • Comparison of EKC with capillary electrochromatography.
  • Emphasis on strategies for improving detection sensitivity, such as on-line concentration.
  • Discussion of on-line coupling with mass spectrometry.

Conclusions:

  • Electrokinetic chromatography, especially MEKC, is a versatile analytical tool.
  • Future research directions include enhancing detection limits and integrating with advanced detection methods like mass spectrometry.