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The use of HPLC for the detection and quantification of formaldehyde in the pteridophyta
M Adrian-Romero1, G Blunden, B G Carpenter
1School of Pharmacy and Biomedical Sciences, University of Portsmouth, UK.
Acta Biologica Hungarica
|October 20, 1999
Abstract:
Formaldehyde, as its dimedone adduct, formaldemethone, has been detected and quantified in all species of Pteridophyta examined. The procedure involved the use of an Hypersil C-18 column, methanol-water (60 : 40 v/v) as the mobile phase and an UV detector set at 258 nm. Quantification was based on peak height. Yields varied from 30 microg/g fresh weight for Polystichum setiferum to 5370 microg/g fresh weight for Selaginella viticulosa.