Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structure activity studies on somatostatin.

D Sarantakis, W A McKinley, I Jaunakais

    Clinical Endocrinology
    |January 1, 1976
    PubMed
    Summary

    Synthetic somatostatin analogues reveal key structural needs for inhibiting growth hormone release in rats. Specific modifications show that the peptide ring size is not critical for maintaining biological activity.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Search for Gluino-Mediated Bottom Squark Production in pp[over ] Collisions at sqrt[s]=1.96 TeV.

    Physical review letters·2009
    Same author

    Search for exclusive Z-boson production and observation of high-mass pp[over ]-->pgammagammap[over ]-->pl;{+}l;{-}p[over ] events in pp[over ] collisions at sqrt[s]=1.96 TeV.

    Physical review letters·2009
    Same author

    First measurement of the tt[over ] differential cross section dsigma/dM_{tt[over ]} in pp[over ] collisions at sqrt[s]=1.96 TeV.

    Physical review letters·2009
    Same author

    Patients' satisfaction with the nurse-led aural care clinic.

    Journal of Ayub Medical College, Abbottabad : JAMC·2009
    Same author

    Apolipoprotein-E gene variants associated with cardiovascular risk factors in antipsychotic recipients.

    European psychiatry : the journal of the Association of European Psychiatrists·2009
    Same author

    Search for top-quark production via flavor-changing neutral currents in W+1 jet events at CDF.

    Physical review letters·2009

    Area of Science:

    • Endocrinology
    • Pharmacology
    • Biochemistry

    Background:

    • Somatostatin is a peptide hormone with diverse physiological roles, including the regulation of growth hormone secretion.
    • Understanding the structure-activity relationship of somatostatin analogues is crucial for developing targeted therapeutics.

    Purpose of the Study:

    • To investigate the structural requirements of somatostatin for suppressing growth hormone secretion in rats.
    • To evaluate the impact of specific amino acid deletions on the biological activity of somatostatin analogues.

    Main Methods:

    • Synthesis and characterization of seven novel somatostatin analogues.
    • In vivo studies in rats to measure plasma levels of growth hormone, insulin, and glucagon following analogue administration.

    Main Results:

    • The size of the somatostatin ring is not essential for maintaining activity.
    • Peptides with deletions at serine-13, lysine-4, or asparagine-5 retained significant biological activity.
    • A specific analogue, des-Ala1, Gly2, Asn5-somatostatin, effectively lowered plasma growth hormone and insulin without significantly altering glucagon levels.

    Conclusions:

    • Structural modifications, particularly deletions of specific amino acids, can yield somatostatin analogues with retained or altered biological functions.
    • The analogue des-Ala1, Gly2, Asn5-somatostatin represents a promising candidate for further investigation due to its selective effects on hormone levels.

    Related Experiment Videos