Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A dissolution test for a pressure-controlled colon delivery capsule: rotating beads method.

Y Yoshikawa1, Z Hu, G Kimura

  • 1Department of Pharmacokinetics, Kyoto Pharmaceutical University, Japan.

The Journal of Pharmacy and Pharmacology
|October 21, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Uric acid levels predict future blood pressure and new onset hypertension in the general Japanese population.

Journal of human hypertension·2014
Same author

A randomized multicenter phase II trial on the efficacy of a hydrocolloid dressing containing ceramide with a low-friction external surface for hand-foot skin reaction caused by sorafenib in patients with renal cell carcinoma.

Annals of oncology : official journal of the European Society for Medical Oncology·2013
Same author

Factors associated with brachial-ankle pulse wave velocity in the general population.

Journal of human hypertension·2011
Same author

Cytotoxic and cytostatic effects of polyphenols against rat 3y1 fibroblasts transformed by e1a gene of human adenovirus-type-12.

International journal of oncology·2011
Same author

Reversible proliferation arrest of rat 3y1 fibroblasts and selective killing of simian-virus transformed derivation of 3y1 by short-chain Fatty-acids.

International journal of oncology·2011
Same author

E1a-3y1 cell-specific toxicity of tea polyphenols and their killing mechanism.

International journal of oncology·2011

A new rotating beads method effectively differentiates drug release from pressure-controlled colon delivery capsules (PCDCs) and enteric-coated tablets. This in-vitro test correlates well with in-vivo drug absorption, validating its use for colon drug delivery systems.

Area of Science:

  • Pharmaceutical Sciences
  • Drug Delivery Systems
  • In-vitro testing

Background:

  • Colon drug delivery aims for targeted drug release in the large intestine.
  • Pressure-controlled colon delivery capsules (PCDCs) offer a novel approach for colon-specific drug delivery.
  • Existing in-vitro dissolution tests may not adequately differentiate the performance of various colon delivery systems.

Purpose of the Study:

  • To introduce and optimize a novel in-vitro dissolution testing method, the rotating beads method.
  • To evaluate the method's ability to distinguish dissolution profiles between PCDCs and conventional enteric-coated tablets.
  • To assess the correlation between in-vitro dissolution data and in-vivo drug absorption.

Main Methods:

  • The rotating beads method utilized a glass vessel with rotating glass beads and a simulated colonic fluid (phosphate buffer with polyvinyl alcohol).

Related Experiment Videos

  • Fluorescein was used as a model drug in PCDCs and Eudragit S-100 coated tablets.
  • Optimized conditions involved 10,000 beads, 50 mL of 10% PVA solution, and a rotation speed of 10 rev min⁻¹.

    • Main Results:

    • The rotating beads method successfully differentiated fluorescein dissolution between PCDCs and enteric-coated tablets under optimized conditions.
    • The method was also applied to acetaminophen, tegafur, and 5-aminosalicylic acid, showing significant differences in drug release.
    • A good correlation was observed between in-vitro dissolution parameters (T50) and in-vivo absorption parameters (Cmax/Tmax or Cmax/(Tmax-Ti)).

    Conclusions:

    • The rotating beads method is a valuable and sensitive in-vitro technique for evaluating colon drug delivery systems, particularly PCDCs.
    • This method provides a reliable prediction of in-vivo drug absorption, aiding in the development of effective colon-targeted therapies.
    • The optimized conditions enable robust discrimination between different colon delivery formulations.