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Solid immunoassay and immunonephelemetric methods for serum C reactive protein (CRP) determination: discrepancy in

M Lelong1, M F Renard, V Giraudeaux

  • 1Service de biochimie et biologie moléculaire, Centre de recherche Claude Bernard, IFR 6, Hopital Lariboisière, 2 rue Ambroise Paré 75010, Paris, France.

Clinica Chimica Acta; International Journal of Clinical Chemistry
|October 26, 1999
PubMed
Abstract

No abstract available in PubMed .

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In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.