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Related Experiment Videos

The cII locus in the MutaMouse system.

R R Swiger1, L Cosentino, N Shima

  • 1Department of Biology, York University, Toronto, Ontario M3J 1P3 Canada.

Environmental and Molecular Mutagenesis
|October 26, 1999
PubMed
Summary

The cII locus is now characterized for the MutaMouse system, enabling efficient in vivo somatic mutation quantification. This new target offers advantages for genetic mutation studies in mice.

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Area of Science:

  • Genetics
  • Toxicology
  • Molecular Biology

Background:

  • The MutaMouse system is widely used for in vivo somatic mutation quantification.
  • The lacZ gene, commonly used in MutaMouse, presents challenges for efficient sequence analysis due to its large size.
  • A need exists for alternative, more easily analyzed mutational targets.

Purpose of the Study:

  • To report the first application and characterization of the cII locus as a mutational target in the MutaMouse system.
  • To compare the performance of the cII locus with the lacZ gene in terms of spontaneous and induced mutation frequencies.
  • To evaluate the suitability of the cII locus for efficient sequence spectra analysis and jackpot mutation identification.

Main Methods:

  • Application and characterization of the cII locus in the MutaMouse system.

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  • Analysis of mutations using positive selection.
  • Treatment of MutaMice with N-ethyl-N-nitrosourea (ENU) at various doses.
  • Comparison of mutation frequencies at cII and lacZ loci.

    • Main Results:

    • The cII locus demonstrated similar spontaneous and induced mutation frequencies compared to the lacZ gene.
    • Mutant frequencies at the cII locus reached a maximum 10 days post-ENU treatment and remained constant.
    • The cII locus showed a linear dose-response to ENU treatment.
    • The smaller size of the cII locus (0.3 kb) facilitates efficient sequence spectra analysis compared to the lacZ gene (3.0 kb).

    Conclusions:

    • The cII locus is a genetically neutral and effective mutational target for the MutaMouse system.
    • The cII locus offers improved efficiency for sequence analysis and jackpot mutation identification.
    • The cII locus provides a common transgenic target for both MutaMouse and Big Blue in vivo mutagenesis assays.