Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Lymphocyte subpopulations in full-term septic neonates.

U Godula-Stuglik1, B Mazur, G Mikusz

  • 1Department of Neonatal Intensive Care, Silesian School of Medicine, Zabrze, Poland. Norsha@domnet.zabrze.pl

Pediatrics International : Official Journal of the Japan Pediatric Society
|October 26, 1999
PubMed
Summary

Septic neonates show altered T-lymphocyte subsets, with increased CD3+ and CD3+/CD4+ T cells and a higher CD4+/CD8+ ratio, indicating changes in cell-mediated immunity. Perinatal factors did not significantly influence these lymphocyte counts.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Food dependent activation of one carbon metabolism during heat shock in Mytilus californianus.

The Journal of experimental biology·2026
Same author

Lower serum B12 vitamin concentrations are not accompanied by hyperhomocysteinaemia in chronic spontaneous urticaria.

Clinical and experimental dermatology·2016
Same author

Pentraxin-3 as a local inflammatory marker in chronic spontaneous urticaria.

Cytokine·2015
Same author

The immunophenotypes of blast cells in B-cell precursor acute lymphoblastic leukemia: how different are they from their normal counterparts?

Cytometry. Part B, Clinical cytometry·2014
Same author

The immunophenotypes of blast cells in B-cell precursor acute lymphoblastic leukemia: How different are they from their normal counterparts?

Cytometry. Part B, Clinical cytometry·2014
Same author

Interleukin 1-β, interleukin-1 receptor antagonist, and interleukin 18 in children with acute spontaneous urticaria.

BioMed research international·2014

Area of Science:

  • Immunology
  • Neonatal Medicine
  • Cell Biology

Background:

  • Neonatal sepsis is a critical condition affecting newborns.
  • Understanding immune system changes in septic neonates is crucial for treatment.
  • Lymphocyte subset analysis provides insights into cell-mediated immunity.

Purpose of the Study:

  • To quantify leukocyte and lymphocyte subsets in term septic neonates.
  • To assess relative and absolute sizes of B and T lymphocytes (CD19+, CD3+, CD4+, CD8+, CD3+/HLA-DR+).
  • To investigate the impact of perinatal risk factors on lymphocyte subsets in neonatal sepsis.

Main Methods:

  • Investigated 21 septic and 15 healthy full-term neonates.
  • Utilized two-color flow cytometric immunophenotyping on lysed whole blood.

Related Experiment Videos

  • Analyzed specific lymphocyte subpopulations including B cells and various T cell subsets.

    • Main Results:

    • Septic neonates had significantly higher relative sizes of CD3+ and CD3+/CD4+ T lymphocytes and an elevated CD4+/CD8+ ratio compared to controls.
    • Absolute counts of CD4+ T cells were significantly higher in septic neonates.
    • No significant differences were observed in CD19+ B lymphocytes, CD3+/CD8+, or CD3+/HLA-DR+ T lymphocytes between groups.
    • Perinatal risk factors did not significantly affect lymphocyte counts in septic neonates.

    Conclusions:

    • Elevated relative sizes of CD3+ and CD3+/CD4+ T lymphocytes and the CD4+/CD8+ ratio in septic neonates suggest altered cell-mediated immunity.
    • Findings provide valuable information on early neonatal immune responses during sepsis.
    • Further research may elucidate the clinical implications of these immune changes.