Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Endotoxin activity measured by limulus assay.

E Haight-Ponce1, H Endo, H Horiuchi

  • 1Department of Endodontics and Periodontics, Tohoku University School of Dentistry, Sendai, Japan. eleanor@mail.cc.tohoku.ac.jp

Endodontics & Dental Traumatology
|October 26, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Perioperative management of cerebral circulation].

Masui. The Japanese journal of anesthesiology·1998
Same author

Production and immunohistochemical characterization of monoclonal antibodies against ovarian antigen of the common tree shrew (Tupaia glis).

Anatomia, histologia, embryologia·1998
Same author

[Home care problems of new long-term care insurance in Japan].

Nihon Ronen Igakkai zasshi. Japanese journal of geriatrics·1998
Same author

Geographical variation of mandible size and shape in the Ryukyu wild pig (Sus scrofa riukiuanus).

The Journal of veterinary medical science·1998
Same author

Functional anatomy of gliding membrane muscles in the sugar glider (Petaurus breviceps).

Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft·1998
Same author

Sensory feedback contributes to early movement-evoked fields during voluntary finger movements in humans.

Brain research·1997

Sodium hypochlorite significantly reduced endotoxin binding to dentin powder compared to hydrogen peroxide. This effect was most pronounced at 1 and 10 minutes, with the lowest binding observed after 30 minutes.

Area of Science:

  • Biomaterials Science
  • Microbiology
  • Dental Materials

Background:

  • Endotoxins, such as lipopolysaccharides from Escherichia coli, are potent inflammatory agents found in dental environments.
  • Dentin, a primary tooth tissue, can interact with and bind endotoxins, potentially influencing inflammatory responses.
  • Pre-treatment of dentin with irrigating solutions may alter its capacity to bind endotoxins.

Purpose of the Study:

  • To investigate the effect of sodium hypochlorite and hydrogen peroxide on endotoxin binding to dentin powder.
  • To compare the binding activity of endotoxin to dentin pre-treated with different concentrations and durations of irrigating solutions.

Main Methods:

  • Dentin powder was pre-treated with 10% sodium hypochlorite or 3% hydrogen peroxide for 1, 10, and 30 minutes.

Related Experiment Videos

  • Endotoxin (Escherichia coli 0111) solutions at 100 ng/ml were incubated with the treated dentin powder.
  • The Limulus amoebocyte lysate (LAL) test was employed to quantify endotoxin binding levels using a microtiter plate reader.

    • Main Results:

    • Endotoxin binding activity was significantly lower in dentin treated with 10% sodium hypochlorite compared to 3% hydrogen peroxide at 1-minute and 10-minute intervals.
    • The lowest endotoxin binding activity was observed in the 30-minute test period, irrespective of the pre-treatment agent.
    • Dentin pre-treatment demonstrably affects endotoxin binding capacity.

    Conclusions:

    • 10% sodium hypochlorite is more effective than 3% hydrogen peroxide in reducing endotoxin binding to dentin powder.
    • The duration of pre-treatment influences the extent of endotoxin binding reduction.
    • These findings have implications for endodontic disinfection protocols and managing inflammatory responses in dental treatments.