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Expression of adhesion molecules on synovial fluid and peripheral blood monocytes in patients with inflammatory joint

M Köller1, M Aringer, H Kiener

  • 1Division of Rheumatology, Department of Internal Medicine III, University of Vienna, Währinger Gürtel 18-20, A-1090 Vienna, Austria.

Annals of the Rheumatic Diseases
|October 26, 1999
PubMed
Abstract

Insights

Monocytes/macrophages (Mphi) in inflammatory joint diseases show increased adhesion molecules in peripheral blood, indicating activation and recruitment to the joint. Synovial fluid Mphi express different adhesion molecules compared to peripheral blood.

Area of Science:

  • Immunology
  • Rheumatology
  • Cell Biology

Background:

  • Osteoarthritis (OA) and inflammatory joint diseases like rheumatoid arthritis (RA) and reactive arthritis (ReA) involve complex cellular mechanisms.
  • Monocytes/macrophages (Mphi) play a crucial role in joint inflammation and tissue remodeling.

Purpose of the Study:

  • To investigate the expression of key adhesion molecules on Mphi from peripheral blood (PB) and synovial fluid (SF).
  • To differentiate Mphi activation states in OA versus inflammatory joint diseases (RA, ReA).
  • To elucidate the role of Mphi adhesion molecules in the pathogenesis of joint inflammation.

Main Methods:

  • Flow cytometry was used for dual fluorescence analysis of Mphi.
  • Monoclonal antibodies targeted adhesion molecules CD11a (LFA-1), CD15s (sialyl-Lewis X), CD44, CD54, VLA-4, and HLA-DR.
  • Anti-CD14 antibodies identified Mphi in both PB and SF samples.

Main Results:

  • CD15s expression was significantly higher on PB-Mphi in RA and ReA compared to OA.
  • PB-Mphi in ReA showed increased LFA-1, CD44, and HLA-DR surface density versus OA.
  • SF-Mphi exhibited significantly higher CD44 and CD54 expression than PB-Mphi.

Conclusions:

  • Mphi in the peripheral blood of patients with inflammatory joint diseases are activated, facilitating their recruitment into the synovial compartment.
  • Inflammatory joint diseases (RA, ReA) appear to have a systemic component, unlike OA.
  • Distinct adhesion molecule profiles exist between SF-Mphi and PB-Mphi in CD14(+) cells.

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