Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Granulocyte storage and antigen stability.

H Chun1, K Cipolone, J Procter

  • 1Department of Transfusion Medicine, Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892-1184, USA. hchun@dtm.cc.nih.gov

Transfusion
|October 26, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Updated assessment of occupational safety and health hazards of climate change.

Journal of occupational and environmental hygiene·2023
Same author

Species and characteristics of volatile organic compounds emitted from an auto-repair painting workshop.

Scientific reports·2021
Same author

Correction: Astrocytic water channel aquaporin-4 modulates brain plasticity in both mice and humans: a potential gliogenetic mechanism underlying language-associated learning.

Molecular psychiatry·2021
Same author

[Interpretation for diagnosis of Yersiniosis].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2019
Same author

Astrocytic water channel aquaporin-4 modulates brain plasticity in both mice and humans: a potential gliogenetic mechanism underlying language-associated learning.

Molecular psychiatry·2018
Same author

Visible light communication using InGaN optical sources with AlInGaP nanomembrane down-converters.

Optics express·2016
Same journal

Investigation of the preanalytical stability of blood donor samples.

Transfusion·2026
Same journal

The need for dried plasma-Still a national issue: Where are we and recommendations.

Transfusion·2026
Same journal

Spray dried plasma manufactured from apheresis and whole blood derived plasma.

Transfusion·2026
Same journal

Identification of a novel ABO*A1.01 allele with c.562C>T (p.Arg188Cys) mutation associated with A<sub>el</sub> phenotype in a Chinese individual.

Transfusion·2026
Same journal

AABB survey on directed blood donation practices.

Transfusion·2026
Same journal

Cost analysis considerations for red blood cell matching to mitigate alloimmunization in patients with sickle cell disease.

Transfusion·2026
See all related articles

Storing granulocytes with formaldehyde or a commercial solution preserves them for 7 days. However, non-specific staining limits antibody detection to 4 days, impacting granulocyte antibody assays.

Area of Science:

  • Immunology
  • Cell Biology
  • Flow Cytometry

Background:

  • Current granulocyte antibody detection methods are labor-intensive and costly.
  • Freshly isolated cells are required, posing logistical challenges.

Purpose of the Study:

  • To develop and evaluate methods for storing granulocytes for antibody detection assays.
  • To improve the efficiency and accessibility of granulocyte antibody testing.

Main Methods:

  • Modified immunofluorescence for flow cytometry.
  • Tested granulocyte storage at 4°C for 7 days using formaldehyde fixation, RPMI-1640, or Cyto-Chex Reagent.
  • Evaluated antigen stability with monoclonal antibodies (MoAbs) and alloantibodies.

Main Results:

Related Experiment Videos

  • Formaldehyde-fixed granulocytes (in HBSS) showed 67-76% viability on Day 7.
  • Commercial storage solution yielded 47-87% viable cells on Day 7.
  • Antigens remained detectable on Day 7, but non-specific staining by FITC conjugates hindered results after Day 4.

Conclusions:

  • Granulocytes and their antigens can be preserved for at least 7 days.
  • Non-specific staining due to increased cell permeability limits reliable antibody detection to 4 days.
  • Optimized storage and detection methods are needed to overcome limitations in granulocyte antibody assays.