Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Protease activity induced by nicotine in human cells.

X L Wang1, K Kita, S Takahashi

  • 1Department of Oral Surgery, Chiba University School of Medicine, Chuo-ku, Chiba 260-8670, Japan.

International Journal of Molecular Medicine
|October 27, 1999
PubMed
Summary

Nicotine exposure can increase fibrinolytic protease activity in human cells. This study identified serine proteases as the key enzymes induced by nicotine, particularly in APr-1 cells.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Response to Letter to the Editor: "Aberrant Collagen Cross-linking in Human Oral Squamous Cell Carcinoma".

Journal of dental research·2019
Same author

Frequency-Domain Multiplexing Readout with a Self-Trigger System for Pulse Signals from Kinetic Inductance Detectors.

Journal of low temperature physics·2019
Same author

Aberrant Collagen Cross-linking in Human Oral Squamous Cell Carcinoma.

Journal of dental research·2019
Same author

Widespread white matter microstructural differences in schizophrenia across 4322 individuals: results from the ENIGMA Schizophrenia DTI Working Group.

Molecular psychiatry·2017
Same author

Prefrontal cortical thinning links to negative symptoms in schizophrenia via the ENIGMA consortium.

Psychological medicine·2017
Same author

Positive symptoms associate with cortical thinning in the superior temporal gyrus via the ENIGMA Schizophrenia consortium.

Acta psychiatrica Scandinavica·2017

Area of Science:

  • Biochemistry
  • Cell Biology
  • Pharmacology

Background:

  • Nicotine exerts diverse biological effects.
  • Proteases play crucial roles in biological processes.
  • Understanding nicotine's impact on protease activity is important.

Purpose of the Study:

  • To investigate if nicotine induces proteolytic protease activity.
  • To identify the specific human cell lines responsive to nicotine-induced protease activity.
  • To characterize the nature of these nicotine-induced proteases.

Main Methods:

  • Assessed plasminogen activator-like fibrinolytic protease activity using 125I-fibrin and plasminogen.
  • Treated 16 human cell lines with and without nicotine.
  • Partially purified induced proteases using HPLC gel filtration (TSKG2000SW).

Related Experiment Videos

  • Utilized protease inhibitors for characterization.

    • Main Results:

    • Nicotine induced significant fibrinolytic protease activity in APr-1 cells, the highest among 16 cell lines tested.
    • Partially purified proteases were obtained via HPLC gel filtration.
    • Protease inhibitor tests revealed the induced proteases are serine proteases.

    Conclusions:

    • Nicotine can induce fibrinolytic protease activity in human cell cultures.
    • APr-1 cells exhibit the highest response to nicotine-induced protease activity.
    • The induced proteases are characterized as serine proteases.