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Quantitative analysis of cimetidine in human plasma using LC/APCI/SRM/MS
K Xu1, V K Arora, A K Chaudhary
1Center for Cancer Pharmacology, Department of Pharmacology, University of Pennsylvania, Philadelphia, PA 19104-4318, USA.
Biomedical Chromatography : BMC
|October 27, 1999
Summary
A new, sensitive method accurately measures cimetidine in human plasma using liquid chromatography-mass spectrometry. This rapid assay requires minimal sample volume and is effective for analyzing drug levels in premature infants.
Area of Science:
- Pharmacology
- Analytical Chemistry
- Clinical Chemistry
Background:
- Cimetidine is a crucial medication, particularly for premature infants.
- Accurate quantification of cimetidine in plasma is essential for therapeutic drug monitoring.
- Existing analytical methods may lack the sensitivity or speed required for clinical applications.
Purpose of the Study:
- To develop and validate a rapid, sensitive, and quantitative method for cimetidine analysis in human plasma.
- To establish a reliable assay for determining cimetidine concentrations in clinical settings, especially in vulnerable populations.
Main Methods:
- Liquid chromatography (LC) coupled with atmospheric pressure chemical ionization (APCI) and selected reaction monitoring (SRM) mass spectrometry (MS).
- Utilized a reverse-phase HPLC C18 column for separation and SKF92374 as an internal standard.
- Validated the method for linearity, precision, and accuracy using human plasma samples.
Main Results:
- Achieved a lower limit of quantitation of 5 ng/mL and linearity up to 10,000 ng/mL.
- Demonstrated excellent precision (1.1-8.9% RSD) and accuracy (94.7-108.0%).
- Required only 50 μL of plasma with efficient sample pretreatment (analyte recovery >94%).
Conclusions:
- The developed LC-APCI-SRM-MS method is highly sensitive, accurate, and rapid for quantifying cimetidine in human plasma.
- This validated assay is suitable for therapeutic drug monitoring, including its application in premature infants.
- The method's efficiency and minimal sample requirement make it valuable for clinical pharmacokinetic studies.