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Developmental expression of Manduca sexta hemolin.

X Q Yu1, M R Kanost

  • 1Department of Biochemistry, Kansas State University, Manhattan, Kansas 66506, USA.

Archives of Insect Biochemistry and Physiology
|October 27, 1999
PubMed
Summary
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Hemolin, an immunoglobulin superfamily protein, is developmentally regulated in Manduca sexta larvae and adults. Its expression increases significantly during the wandering stage and in response to bacterial infection, suggesting a role in immunity.

Area of Science:

  • Insect immunology
  • Molecular biology
  • Developmental biology

Background:

  • Hemolin is a hemolymph protein belonging to the immunoglobulin superfamily.
  • Induced expression after bacterial infection suggests a role in immune response.

Purpose of the Study:

  • Investigate the developmental expression of the Manduca sexta hemolin gene.
  • Determine hemolin's presence and characteristics in naive insects.

Main Methods:

  • Analysis of hemolin gene expression at different developmental stages.
  • Isolation and sequencing of hemolin cDNA.
  • Purification and characterization of hemolin protein from hemolymph and midgut fluid.

Main Results:

  • Hemolin levels increase dramatically in larvae during the wandering stage and are present in midgut lumen.

Related Experiment Videos

  • Hemolin is found in hemolymph throughout pupal and adult stages, and in eggs.
  • Purified hemolin shows consistent mass but variable carbohydrate association depending on developmental stage and infection status.

    • Conclusions:

    • The Manduca sexta hemolin gene is developmentally regulated and induced by microbial infection.
    • Hemolin lacks post-translational covalent glycosylation but can associate with non-covalently bound carbohydrates.