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Characterization of glycosylphosphatidylinositol-linked molecule CD55/decay-accelerating factor as the receptor for

F Hensel1, R Hermann, C Schubert

  • 1Institut für Pathologie, Würzburg, Germany.

Cancer Research
|October 28, 1999
PubMed

Insights

The human monoclonal antibody SC-1 targets a unique stomach cancer cell receptor, a variant of CD55 (decay-accelerating factor). This antibody triggers cancer cell death, offering potential for gastric carcinoma treatment.

Area of Science:

  • Oncology
  • Immunology
  • Molecular Biology

Background:

  • Human monoclonal antibody SC-1 induces apoptosis in stomach carcinoma cells.
  • SC-1 targets a molecule preferentially expressed on diffuse- and intestinal-type stomach cancers with restricted normal tissue expression.

Purpose of the Study:

  • Identify the SC-1 receptor.
  • Elucidate the mechanism of SC-1-induced apoptosis in gastric carcinoma.

Main Methods:

  • Immunoprecipitation and Western blotting to identify the SC-1 receptor.
  • Analysis of protein phosphorylation and caspase activation.
  • Assessment of SC-1 binding and receptor cross-linking effects.

Main Results:

  • The SC-1 receptor is identified as a stomach carcinoma-associated isoform of CD55 (DAF-B), approximately 82 kDa.
  • SC-1's antigenic site is an N-linked carbohydrate residue.
  • SC-1 binding induces tyrosine phosphorylation of specific proteins and dephosphorylation of another, alongside elevated expression and activation of caspases-3 and -8.

Conclusions:

  • A tumor-specific variant of DAF is involved in gastric carcinoma apoptosis.
  • SC-1 shows potential as an adjuvant therapeutic agent for gastric carcinoma.

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