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Flow cytometric analysis of platelet function in stored platelet concentrates
Transfusion Science
|October 28, 1999
Summary
Stored platelets show initial activation and a decline in function over five days. While not progressively activated by storage, their response to stimuli decreases, impacting overall platelet efficacy.
Area of Science:
- Transfusion Medicine
- Hematology
- Cellular Biology
Background:
- Platelet activation is a known consequence of collection, processing, and storage of platelet concentrates.
- The impact of this activation on the functional capacity of stored platelets remains incompletely understood.
Purpose of the Study:
- To assess platelet activation and functional response to thrombin stimulation in stored platelet concentrates.
- To evaluate changes in platelet markers and function over a five-day storage period under routine blood bank conditions.
Main Methods:
- Flow cytometry was used to analyze platelet surface markers (CD62, CD63, GPIb, GPIIbIIIa).
- Mepacrine uptake assay assessed dense granule content.
- Platelet microparticle generation was quantified.
Main Results:
- Platelets exhibited activation by day 1 post-preparation.
- Platelet microparticle generation increased with storage time.
- While initial activation markers did not significantly change, platelet functional reserve (response to thrombin) decreased with storage duration.
Conclusions:
- Platelet concentrates are activated during initial handling and processing.
- Storage does not lead to progressive activation but results in reduced platelet functionality.
- This hypofunctionality may impact the clinical efficacy of stored platelet transfusions.
