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PI-PLC releases a 25-40 kDa protein cluster from the hamster oolemma and affects the sperm penetration assay
S Coonrod1, S Naaby-Hansen, J Shetty
1Center for Recombinant Gamete Contraceptive Vaccinogens, University of Virginia, Charlottesville, VA 22908, USA.
Abstract:
The effects of phosphatidylinositol-specific phospholipase C (PI-PLC) on human sperm-hamster oocyte interaction were investigated to determine whether PI-PLC cleavable glycosylphosphatidyinositol (GPI)-anchored proteins are involved in sperm-egg binding and fusion. Two-dimensional electrophoresis was then utilized to visualize proteins released from hamster oocytes following PI-PLC treatment. For the binding and fusion assay, either spermatozoa or eggs were treated with 1 IU/ml PI-PLC for 30 min and washed prior to gamete co-incubation. Treatment of human spermatozoa with PI-PLC significantly (P