Cloning, expression, and characterization of cadmium and manganese uptake genes from Lactobacillus plantarum

An Mn(2+) and Cd(2+) uptake gene, mntA, was cloned from Lactobacillus plantarum ATCC 14917 into Escherichia coli. Its expression conferred on E. coli cells increased Cd(2+) sensitivity as well as energy-dependent Cd(2+) uptake activity. Both transcription and translation of mntA were induced by Mn(2+) starvation in L. plantarum, as indicated by reverse transcriptase PCR and immunoblotting. Two Cd(2+) uptake systems have been identified in L. plantarum: one is a high-affinity Mn(2+) and Cd(2+) uptake system that is expressed in Mn(2+)-starved cells, and the other is a nonsaturable Cd(2+) uptake system that is expressed in Cd(2+)-sufficient cells (Z. Hao, H. R. Reiske, and D. B. Wilson, Appl. Environ. Microbiol. 65:592-99, 1999). MntA was not detected in an Mn(2+)-dependent mutant of L. plantarum which had lost high-affinity Mn(2+) and Cd(2+) uptake activity. The results suggest that mntA is the gene encoding the high-affinity Mn(2+) and Cd(2+) transporter. On the basis of its predicted amino acid sequence, MntA belongs to the family of P-type cation-translocating ATPases. The topology and potential Mn(2+)- and Cd(2+)-binding sites of MntA are discussed. A second clone containing a low-affinity Cd(2+) transport system was also isolated.