Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Bacterial adhesion at synthetic surfaces.

D Cunliffe1, C A Smart, C Alexander

  • 1Macromolecular Science Department, Institute of Food Research, Reading Laboratory, Reading RG6 6BZ, United Kingdom.

Applied and Environmental Microbiology
|November 5, 1999
PubMed
Summary

Surface chemistry significantly impacts bacterial adhesion. Hydrophilic, uncharged surfaces, particularly those with poly(ethyleneoxide) (PEO) polymers, resist protein and bacterial attachment, suggesting their use for preventing cell adsorption on synthetic materials.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pregnancies in women with Turner syndrome: a retrospective multicentre UK study.

BJOG : an international journal of obstetrics and gynaecology·2021
Same author

PPE consistency please.

British dental journal·2020
Same author

Clarifying the absence of evidence regarding human health risks to microplastic particles in drinking-water: High quality robust data wanted.

Environment international·2020
Same author

GDP referrals.

British dental journal·2020
Same author

Thermoresponsive magnetic colloidal gels via surface-initiated polymerisation from functional microparticles.

Journal of materials chemistry. B·2020
Same author

The electrospinning of a thermo-responsive polymer with peptide conjugates for phenotype support and extracellular matrix production of therapeutically relevant mammalian cells.

Biomaterials science·2020

Area of Science:

  • Biomaterials Science
  • Surface Chemistry
  • Microbiology

Background:

  • Bacterial adhesion to surfaces is a critical factor in biomaterial performance and infection.
  • Understanding the influence of surface physicochemical properties on bacterial attachment is essential for developing advanced materials.

Purpose of the Study:

  • To systematically investigate how surface chemistry, including hydrophilicity, hydrophobicity, chain length, and chemical functionality, affects bacterial adhesion.
  • To assess the role of protein adsorption as an intermediate step in bacterial attachment to modified surfaces.

Main Methods:

  • Grafting functional groups onto glass substrates to create chemically defined surfaces with varying properties.
  • Measuring surface energies and performing protein adsorption experiments using labeled bovine serum albumin and cytochrome c.

Related Experiment Videos

  • Quantifying the adhesion of Listeria monocytogenes, Salmonella typhimurium, Staphylococcus aureus, and Escherichia coli to modified surfaces.

    • Main Results:

    • Hydrophilic, uncharged surfaces demonstrated the highest resistance to protein adsorption.
    • Poly(ethyleneoxide) (PEO) effectiveness was influenced by more than just hydrophilicity and molecular weight.
    • Protein adsorption correlated with short-term bacterial adhesion for most species.
    • L. monocytogenes and E. coli adhesion correlated with substrate chemistry, while S. aureus and S. typhimurium showed different attachment patterns.

    Conclusions:

    • Hydrophilic passivating groups, when uniformly grafted at sufficient density, are effective in preventing bacterial cell adsorption to synthetic substrates.
    • The findings have significant implications for designing biomaterials with reduced fouling and infection potential.
    • Bacterial adhesion mechanisms can vary between species, even on similar surfaces.