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Genotypic diversity among Brevibacillus laterosporus strains
V Zahner1, L Rabinovitch, P Suffys
1Laboratory of Systematic Biochemistry, Department of Biochemistry and Molecular Biology, Oswaldo Cruz Institute-FIOCRUZ, Rio de Janeiro, RJ, Brazil. vzahner@gene.dbbm.fiocruz.br
Abstract:
In comparison with other entomopathogenic Bacillus species, the genome of Brevibacillus laterosporus is poorly characterized. The aim of this study was to examine genetic variability in B. laterosporus by using a range of typing methodologies. Strains of B. laterosporus were examined for variation in 13 chromosomal genes encoding enzymes by multilocus enzyme electrophoresis. Optimal conditions of pulsed-field gel electrophoresis and randomly amplified polymorphic DNA were established that allowed analysis of the genome of B. laterosporus. None of these techniques allowed the identification of a convenient molecular marker for entomopathogenic strains, although one specific primer amplified only DNA from almost all mosquitocidal strains.