Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Immunomarkers for molecular mass.

L V Anderson1

  • 1Neurobiology Department, University Medical School, Newcastle upon Tyne, UK. L.V.B.Anderson@ncl.ac.uk

Neuromuscular Disorders : NMD
|November 2, 1999
PubMed
Summary

Researchers developed a new method using antibody cocktails to create molecular weight markers for Western blots. This technique aids in analyzing the diverse protein sizes associated with muscular dystrophy in human skeletal muscle.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Late onset in dysferlinopathy widens the clinical spectrum.

Neuromuscular disorders : NMD·2008
Same author

Mutations in the fukutin-related protein gene (FKRP) identify limb girdle muscular dystrophy 2I as a milder allelic variant of congenital muscular dystrophy MDC1C.

Human molecular genetics·2001
Same author

Dysferlin protein analysis in limb-girdle muscular dystrophies.

Journal of molecular neuroscience : MN·2001
Same author

The phenotype of calpainopathy: diagnosis based on a multidisciplinary approach.

Neuromuscular disorders : NMD·2001
Same author

Normal calpain expression in genetically confirmed limb-girdle muscular dystrophy type 2A.

Neurology·2001
Same author

Cloning of the mouse dysferlin gene and genomic characterization of the SJL-Dysf mutation.

Neuroreport·2001

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genetics

Background:

  • Muscular dystrophies are a group of inherited muscle-wasting diseases.
  • The causative proteins vary significantly in size.
  • Accurate molecular weight determination is crucial for diagnosis and research.

Purpose of the Study:

  • To develop a reliable method for creating molecular mass markers on Western blots.
  • To facilitate the analysis of proteins involved in various forms of muscular dystrophy.

Main Methods:

  • Utilized a cocktail of antibodies targeting proteins of different molecular weights.
  • Applied this cocktail to generate a ladder of molecular mass markers.
  • Performed Western blot analysis on human skeletal muscle samples.

Main Results:

  • Successfully generated a molecular mass marker ladder on Western blots.
  • Demonstrated the utility of this method for analyzing diverse protein sizes.
  • Provided a tool for better characterization of muscular dystrophy proteins.

Conclusions:

  • The antibody cocktail method is effective for creating molecular mass markers.
  • This technique enhances the analysis of skeletal muscle proteins in muscular dystrophy research.
  • Offers a valuable tool for the study of muscle diseases.

Related Experiment Videos