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A microfabricated fluorescence-activated cell sorter.

A Y Fu1, C Spence, A Scherer

  • 1Department of Applied Physics, California Institute of Technology, Pasadena, CA 91125, USA.

Nature Biotechnology
|November 5, 1999
PubMed
Summary
This summary is machine-generated.

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We developed a disposable microfluidic device for cell sorting, offering higher sensitivity and lower cost than traditional methods. This micro-fluorescence-activated cell sorter (microFACS) successfully sorts fluorescent beads and bacteria while maintaining cell viability.

Area of Science:

  • Biotechnology
  • Microfluidics
  • Analytical Chemistry

Background:

  • Conventional fluorescence-activated cell sorter (FACS) machines are expensive and can lead to cross-contamination.
  • There is a need for more accessible, sensitive, and cost-effective cell sorting technologies.

Purpose of the Study:

  • To demonstrate a disposable microfabricated fluorescence-activated cell sorter (microFACS) for sorting biological entities.
  • To compare the performance of microFACS with conventional FACS machines.

Main Methods:

  • Fabrication of disposable microfluidic chips for cell sorting.
  • Utilizing fluorescence detection for sorting micron-sized fluorescent beads and Escherichia coli cells.
  • Assessing the viability of sorted cells.

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Main Results:

  • MicroFACS chips achieved substantial enrichment of differentially colored fluorescent beads.
  • Successfully separated green fluorescent protein-expressing E. coli from nonfluorescent cells.
  • Sorted bacteria remained viable after extraction from the microFACS device.

Conclusions:

  • The disposable microFACS offers higher sensitivity, no cross-contamination, and lower cost compared to conventional FACS.
  • MicroFACS devices can be used as standalone units or integrated into microanalytical systems.
  • This technology enables efficient and viable sorting of various biological entities.