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Related Experiment Videos

Medicinal yeast extracts.

D J Schlemm1, M J Crowe, R B McNeill

  • 1Department of Biological Sciences, University of Cincinnati, Ohio 45221-0006, USA.

Cell Stress & Chaperones
|November 5, 1999
PubMed
Summary
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Researchers identified specific yeast peptides that significantly accelerate wound healing. These stress-associated proteins, including copper, zinc superoxide-dismutase and acyl-CoA binding protein II, show potent activity in nanomolar concentrations.

Area of Science:

  • Biochemistry
  • Dermatology
  • Molecular Biology

Background:

  • Alcoholic extracts of bakers' yeast (Saccharomyces cerevisiae) have a long history in topical treatments for wounds and burns.
  • Previous research indicated peptides were responsible for therapeutic effects, but specific identification was lacking.

Purpose of the Study:

  • To isolate and identify the specific peptides from Saccharomyces cerevisiae responsible for wound healing.
  • To characterize the activity and potency of these identified peptides.

Main Methods:

  • RPC18 chromatography was used to isolate protein fractions from yeast extract.
  • Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and N-terminal amino acid sequencing were employed for polypeptide identification.

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Main Results:

  • A protein fraction significantly enhanced wound closure in both diabetic and non-diabetic models.
  • The isolated peptides demonstrated activity in nanomolar amounts, exhibiting 600-fold greater potency than the initial extract.
  • Four polypeptides were identified: copper, zinc superoxide-dismutase, ubiquitin, glucose lipid regulated protein (HSP 12), and acyl-CoA binding protein II.

Conclusions:

  • Specific stress-associated proteins from Saccharomyces cerevisiae, including acyl-CoA binding protein II, are key active components in wound healing.
  • These identified peptides represent a highly potent therapeutic agent for wound management.