Related Experiment Video

Updated: Aug 18, 2026

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro
08:53

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

Published on: January 11, 2017

Fission yeast Msp1 is a mitochondrial dynamin-related protein

L Pelloquin1, P Belenguer, Y Menon

  • 1Laboratoire de Biologie Cellulaire et Moléculaire du Contrôle de la Prolifération, Université Paul Sabatier, CNRS EP2079, 31062 Toulouse cedex, France.

Journal of Cell Science
|November 5, 1999
PubMed

Insights

Msp1p, a fission yeast mitochondrial protein, is crucial for maintaining mitochondrial DNA. Its targeted import and localization within mitochondria highlight its essential role in mitochondrial structure and function.

Area of Science:

  • Cell Biology
  • Mitochondrial Biology
  • Molecular Genetics

Background:

  • Mitochondrial DNA maintenance is vital for cellular function.
  • Dynamin-related proteins play diverse cellular roles.
  • The fission yeast Schizosaccharomyces pombe serves as a model organism.

Purpose of the Study:

  • To validate Msp1p as a mitochondrial protein.
  • To elucidate the mechanism of Msp1p import and localization.
  • To investigate the function of Msp1p in mitochondrial maintenance.

Main Methods:

  • In vitro import assays using recombinant mitochondrial processing peptidase.
  • In vivo studies including immunofluorescence and electron microscopy.
  • Mitochondrial enrichment, fractionation, and chimera analysis.

Main Results:

  • Msp1p targeting sequence is processed by mitochondrial processing peptidase.
  • Msp1p is imported into S. pombe mitochondria and localized to the inner membrane matrix side.
  • Overexpression of Msp1p leads to significant mitochondrial structural and functional alterations.

Conclusions:

  • Msp1p is a bona fide mitochondrial protein essential for mitochondrial DNA maintenance.
  • The N-terminal 109 residues of Msp1p contain a functional mitochondrial targeting signal.
  • Msp1p plays a critical role in maintaining mitochondrial integrity and function in S. pombe.

More Related Videos

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy12:04

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy

Published on: June 24, 2019

Analysis of the Expression and Complexes Assembly of the Mitochondrial Respiratory Chain Proteins in the Fission Yeast Schizosaccharomyces pombe08:07

Analysis of the Expression and Complexes Assembly of the Mitochondrial Respiratory Chain Proteins in the Fission Yeast Schizosaccharomyces pombe

Published on: May 2, 2025

Related Concept Videos

Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Microtubule Associated Proteins (MAPs)01:42

Microtubule Associated Proteins (MAPs)

Microtubule function and architecture are regulated by an array of specialized proteins called microtubule-associated proteins or MAPs. These proteins are widespread across different organisms and have conserved protein motifs, like the multi-TOG domain for tubulin binding found in the CLASP family of MAPs. Some MAPs are lineage-specific based on their conserved domains. Their functions depend upon the cytoskeletal architecture and cell type they are located within. In-plant cells, a specific...
Microtubule Associated Motor Proteins01:32

Microtubule Associated Motor Proteins

Eukaryotic cells have different motor proteins for transporting various cargo within the cell. These motor proteins differ based on the filament they associate with, the direction they move within the cell, and the type of cargo they transport. Motor proteins that associate with microtubules are known as microtubule-associated motor proteins. There are two families of microtubule-associated motor proteins —Kinesins and Dyneins. Both these proteins assist in the transport of cellular cargos...
Translocation of Proteins into the Mitochondria01:19

Translocation of Proteins into the Mitochondria

Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
SNAREs and Membrane Fusion01:43

SNAREs and Membrane Fusion

Once a transport vesicle has recognized its target organelle, the vesicular membrane needs to fuse with the target membrane to unload the cargo. Transmembrane proteins called SNAREs present on organelle membranes and their vesicles, mediate vesicle fusion.
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
Fungal Phylum Microsporidia01:28

Fungal Phylum Microsporidia

Microsporidia are a group of obligate intracellular fungi that were initially classified as protists but were later reclassified based on phylogenetic, molecular, and structural evidence linking them to the Chytridiomycota. These unicellular, non-motile organisms are highly specialized parasites that infect a wide range of animal hosts, including humans. They have evolved extensive genomic and metabolic reductions, making them highly dependent on their hosts for survival.Morphology and Genomic...