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Does storage affect epifluorescence microscopic counts of total bacteria in freshwater samples?
F Garabétian1, M Petit, P Lavandier
1Centre d'écologie des systèmes aquatiques continentaux, UMR CNRS C5576-université Paul-Sabatier, Toulouse, France. garabet@mail.cict.fr
Abstract:
Preservation with formalin and storage at 4 degrees C of freshwater samples, associated with sonication before counting, proved to be efficient for a few days of storage (no differences with the initial cell count within 7 d of storage); after 8 months, 95% of the initial count was preserved. This procedure always gave higher counts than those obtained from the freezing technique of filter storage at -18 degrees C after sample filtration.