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Light and electron microscopic studies on alpha naphthyl acetate esterase activity of peripheral blood T lymphocytes
1Department of Histology and Embryology, Faculty of Veterinary Medicine, University of Ankara, Turkey.
Abstract:
The purpose of this study was to determine the percentage of peripheral blood T lymphocytes and the localization of Alpha Naphthyl Acetate Esterase (ANAE) enzyme at the electron microscopic level in healthy adult chickens by an alpha naphthyl acetate esterase procedure. Peripheral blood samples taken from 30 adult Leghorn breed chickens were used. The percentage of ANAE positive lymphocytes was 56.4 +/- 2.82% (SD). Pseudoeosinophil and eosinophil granulocytes showed a granular positivity. In the electron microscopic examination, ANAE positive reactions were seen in lysosomal granules found in T lymphocytes, pseudoeosinophil, and eosinophil granulocytes.