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Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells
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Basic fibroblast growth factor inhibits osteoclast formation induced by 1alpha,25-dihydroxyvitamin D(3) through

N Nakagawa1, H Yasuda, K Yano

  • 1Research Institute of Life Science, Snow Brand Milk Products Co. Ltd. , Tochigi, 329-0512, Japan.

Biochemical and Biophysical Research Communications
|November 5, 1999
PubMed

Insights

Basic fibroblast growth factor (bFGF) inhibits osteoclast formation by reducing osteoclast differentiation factor (ODF) in bone cells. This finding reveals a new mechanism for regulating bone resorption.

Area of Science:

  • Cell Biology
  • Bone Biology
  • Endocrinology

Background:

  • Osteoclast-like cell (OCL) formation is crucial for bone remodeling.
  • 1alpha, 25-dihydroxyvitamin D(3) [1,25(OH)(2)D(3)] is a key inducer of OCL formation.
  • Basic fibroblast growth factor (bFGF) is a signaling molecule with diverse cellular effects.

Purpose of the Study:

  • To investigate the effect of bFGF on OCL formation.
  • To elucidate the mechanism by which bFGF influences OCL differentiation.
  • To determine the role of osteoclast differentiation factor (ODF) and osteoclastogenesis inhibitory factor (OCIF) in bFGF-mediated inhibition.

Main Methods:

  • Coculture of mouse spleen cells with osteoblasts or ST2 stromal cells.
  • Treatment with 1,25(OH)(2)D(3), dexamethasone, and bFGF.
  • Measurement of OCL formation.
  • Analysis of ODF and OCIF mRNA expression using RT-PCR.
  • Enzyme-linked immunosorbent assay (ELISA) for OCIF production.
  • Functional assays using soluble ODF and anti-OCIF antibodies.
  • Binding assays for OCIF.

Main Results:

  • bFGF inhibited 1,25(OH)(2)D(3)-induced OCL formation.
  • bFGF acted directly on osteoblasts/stromal cells, not osteoclast progenitors.
  • bFGF suppressed ODF mRNA expression but did not affect OCIF mRNA or production.
  • Soluble ODF, but not anti-OCIF antibody, abolished bFGF's inhibitory effect.
  • bFGF reduced the binding of OCIF to osteoblasts and ST2 cells.

Conclusions:

  • bFGF inhibits 1,25(OH)(2)D(3)-induced OCL formation.
  • The mechanism involves the suppression of ODF production by osteoblasts/stromal cells.
  • bFGF's action is independent of OCIF production and binding.

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